rabbit polyclonal antibodies against desmin  (ABclonal Biotechnology)

Journal: Biomolecules

Article Title: Young and Undamaged rMSA Improves the Healthspan and Lifespan of Mice

doi: 10.3390/biom11081191

Figure Lengend Snippet: Effects of rMSA injection on the fibrosis level of kidney, liver and heart ( a ) The representative images of α-SMA in mice kidney. Scale bar, 50 μm. ( b , c ) The quantitative results of α-SMA level in female ( b ) and male ( c ) mice. ( d ) The Masson’s trichrome staining of mice kidney. Scale bar, 50 μm. ( e , f ) The collagen volume fraction of the kidney in female ( e ) and male ( f ) mice. ( g ) The immunohistochemical staining for collagen I in mice kidney. Scale bar, 50 μm. ( h , i ) The relative level of collagen I in female ( h ) and male ( i ) mice. ( j ) The immunohistochemical staining for α-SMA in mice liver. Scale bar, 50 μm. ( k , l ) The relative level of α-SMA in female ( k ) and male ( l ) mice. ( m ) The immunohistochemical staining for desmin in mice liver. Scale bar, 50 μm. ( n , o ) The relative level of desmin in female ( n ) and male ( o ) mice. ( p ) The Masson’s trichrome staining of mice liver. Scale bar, 50 μm. ( q , r ) The collagen volume fraction of the liver in female ( q ) and male ( r ) mice. ( s ) The immunohistochemical staining for α-SMA in mice heart. Scale bar, 50 μm. ( t , u ) The relative level of α-SMA in female ( t ) and male ( u ) mice. ( v ) The Masson’s trichrome staining of mice cardiac muscle. Scale bar, 50 μm. ( w , x ) The collagen volume fraction of the cardiac muscle in female ( w ) and male ( x ) mice. All graphs represent mean with SEM, with p values calculated by the two-tail t test. n, number of mice used for each analysis.

Article Snippet: Cell Signaling Technology, Danvers, MA, USA, 91144), rabbit polyclonal antibody against desmin (UniProtKB P31001.

Techniques: Injection, Staining, Immunohistochemical staining

Journal: International Journal of Molecular Sciences

Article Title: MiR-30a Inhibits the Epithelial—Mesenchymal Transition of Podocytes through Downregulation of NFATc3

doi: 10.3390/ijms161024032

Figure Lengend Snippet: MiR-30a is Downregulated in Podocyte Injury. ( A ) RT-qPCR analysis of miR-30a expression levels in glomeruli from BALB/c mouse after Adriamycin (ADR; 10.5 mg/kg) injection for 4, 7, 11, 15 and 20 days ( n = 3, mean ± SD); ( B ) Western blot analysis of Nephrin and Desmin expression levels in cultured MPC5 cells treated with ADR at different doses (2.5, 5.0 and 10.0 µg/mL); Quantitative analysis of Nephrin and Desmin protein levels after normalization with β-tubulin; ( C ) RT-qPCR analysis of miR-30a expression levels in cultured MPC5 cells treated with ADR (5.0 µg/mL) at different time points (0.5, 2, 6, 12 and 24 h); ( D ) RT-qPCR analysis of miR-30a expression levels in the glomeruli from one patient with focal segmental glomerulosclerosis (FSGS) and one patient with kidney rupture (Control). The relative amount of miR-30a was normalized to U6. All data are presented as means ± SD from three independent experiments. * p < 0.05, ** p < 0.01, indicate statistically significant and highly statistically significant differences, respectively.

Article Snippet: The blots were probed with rabbit polyclonal antibody against desmin (Santa Cruz Biotechnology, Santa Cruz, CA, USA; 1:1000), nephrin (Abcam, Cambridge, MA, USA; 1:2000), E-cadherin (Santa Cruz Biotechnology; 1:1000), NFATc3 (Bioss, Beijing, China; 1:500) and mouse monoclonal anti-beta-tubulin (Santa Cruz Biotechnology; 1:3000, anti-beta-actin (Santa Cruz Biotechnology; 1:3000) overnight at 4 °C.

Techniques: Quantitative RT-PCR, Expressing, Injection, Western Blot, Cell Culture, Control